General Cell Based Assays
Eurofins Discoverx PathHunter AGTR1 Bioassay Kit, 10-Plate. (93-0312Y2-00126)
The PathHunter AGTR1 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. Angiotensin-II) based activation of AGTR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTR1 and EA-tagged β-Arrestin-2. Activation of AGTR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Meso Scale Discovery U-PLEX Human IL-17D ASY
The Meso Scale Discovery U-PLEX Human IL-17D Assay Kit is a multiplex immunoassay that measures IL-17D levels in human serum plasma and cell culture supernatant samples The assay uses electrochemiluminescence detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent labels calibrators and diluents needed to perform the assay The kit can quantitatively measure IL-17D levels with high precision and accuracy It is for research use only
Eurofins Discoverx cAMP Hunter eXpress ADORA2B CHO-K1 GPCR Assay, 10-Plate Kit. (95-0041E2CP2L)
The cAMP Hunter ADORA2B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. NECA) based activation of ADORA2B GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADORA2B, a naturally Gαs-coupled GPCR. Upon activation of ADORA2B, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCCR3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0522E2MCP0M)
The PathHunter mCCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL24) based activation of mCCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR3 and EA-tagged β-Arrestin-2. Activation of mCCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR45 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0374E2ACP1M)
The PathHunter GPR45 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR45 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR45 and EA-tagged β-Arrestin-2. Activation of GPR45 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human PC9/PCSK9 ASY
The R-PLEX Human PC9/PCSK9 Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay that simultaneously measures Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Progranulin (PC9) in human serum plasma and cell culture supernatants This electrochemiluminescence (ECL) based assay utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and reproducible quantitation of PCSK9 and PC9 levels from a single small-volume sample With a broad dynamic range and rapid protocol this assay kit allows high-throughput profiling of PCSK9 and PC9 biomarkers involved in lipid metabolism and inflammation
Eurofins Discoverx PathHunter eXpress MTNR1B CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0630E2CP0M)
The PathHunter MTNR1B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. melatonin) based activation of MTNR1B GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MTNR1B and EA-tagged β-Arrestin-2. Activation of MTNR1B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rDRD1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0620E2CP2L)
The PathHunter rDRD eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of rDRD GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rDRD and EA-tagged β-Arrestin-2. Activation of rDRD stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress SSTR5 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0402E3CP0L)
The PathHunter SSTR5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin) based activation of SSTR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR5 and EA-tagged β-Arrestin-2. Activation of SSTR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NPY1R CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0397E2CP0L)
The PathHunter NPY1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. peptide YY) based activation of NPY1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPY1R and EA-tagged β-Arrestin-2. Activation of NPY1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mEBI2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0953E2MCP0M)
The PathHunter mEBI2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dihydroxycholesterol) based activation of mEBI2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEBI2 and EA-tagged β-Arrestin-2. Activation of mEBI2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rVIPR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0913E2RCP2M)
The PathHunter rVIPR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. VIP) based activation of rVIPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rVIPR1 and EA-tagged β-Arrestin-2. Activation of rVIPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress VIPR2 Total Internalization Assay, 2pl Kit. (93-0824E3CP5M)
The PathHunter VIPR2 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of VIPR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged VIPR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the VIPR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter MCHR1 Bioassay Kit, 2-Plate. (93-0940Y3-00149)
The PathHunter MCHR1 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell-based assay to measure drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed for a complete assay, including cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure assay reproducibility and faster implementation from characterization to lot release. This bioassay has been optimized for a 96-well plate format. The protocol can be optimized further for running the assay in a high-throughput 384-well plate format. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx cAMP Hunter eXpress CRHR2 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0048E2CP2M)
The cAMP Hunter CRHR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CRH, sauvagine) based activation of CRHR2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CRHR2, a naturally Gs coupled GPCR. Upon activation of CRHR2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.